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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo

Fig. 1

Exploration of optimal US parameters for reversing MDR in vitro. a Cell viability in MCF-7/ADR and HUVEC cells in 24 h after US exposure with different acoustic intensities; N = 3; *P < 0.05 vs. 0 W/cm2 in MCF-7/ADR cells; (b) Cytotoxicity of ADM alone in the MCF-7/ADR and HUVEC cells; N = 3, *P < 0.05 compared with HUVEC cells; (c) Intracellular ADM concentration in MCF-7/ADR and HUVEC cells in 24 h after US exposure with different acoustic intensities; N = 3; *P < 0.05 vs. 0 W/cm2 in MCF-7/ADR cells; (d) Cytotoxicity of ADM in MCF-7/ADR and HUVEC cells post US+ADM treatment with different acoustic intensities; N = 3; *P < 0.05 vs. 0 W/cm2 in MCF-7/ADR cells; data are represented as mean ± s.d; (e) Images of intracellular ADM distribution in MCF-7/ADR cells post US+ADM treatment with different acoustic intensities (scale bar = 10 μm); (f) Ultrasound acoustic intensity of 0.74 W/cm2 enhanced intracellular ADM uptake and ADM nuclei localization in HEPG2/ADM cells; (g) Cytotoxicity of US+ADM or ADM alone in HEPG2/ADM cells; N = 3; data are represented as mean ± s.d; *P < 0.05; (h) EDU staining and quantification of the proliferative cells in MCF-7/ADR and HEPG2/ADM cells post-treatment with US+ADM or ADM alone (scale bar = 50 μm); N = 3; data are represented as mean ± s.d; *P < 0.05; (i) TUNEL staining and quantification of the apoptotic cells in MCF-7/ADR and HEPG2/ADM cells post-treatment with US+ADM or ADM alone (scale bar = 50 μm); N = 3; data are represented as mean ± s.d; *P < 0.05

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