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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo

Fig. 4

miR-200c/34a participate in US decreasing P-gp expression. a Q-PCR of oxidative stress responsive miRNAs in MCF-7/ADR cells in 24 h after US exposure; N = 3; data are represented as mean ± s.d; *P < 0.05; (b-c) Pre-treated with 5 mM NAC abrogated US-induced miR-200c (b) or miR-34a-3p (c) overexpression in MCF-7/ADR cells; N = 4; data are represented as mean ± s.d; *P < 0.05 compared with CON group; (d) MiR-200c/34a expression in MCF-7/ADR cells treated with 400 mM H2O2; N = 3; data are represented as mean ± s.d; *P < 0.05 compared with CON group; (e) P-gp expression in MCF-7/ADR cells transfected with miR-200c mimic; N = 3; data are represented as mean ± s.d; *P < 0.05; (f) Predicted miR-34a-3p seed sequence match to the sequence in the 3’ UTR of P-gp (ABCB1) mRNA; (g) Verification of ABCB1 as a target gene of miR-34a-3p by the dual luciferase reporter assay; (h) P-gp expression in MCF-7/ADR cells transfected with miR-200c mimic; N = 3; data are represented as mean ± s.d; *P < 0.05; (i-j) MiR-200c (i) or miR-34a-3p (j) inhibition attenuated US-induced P-gp up-regulation in MCF-7/ADR cells; N = 3; data are represented as mean ± s.d; *P < 0.05 compared with anta-NC group; (k) Images of intracellular ADM distribution (Upper, scale bar = 10 μm) and TUNEL staining (lower, scale bar = 50 μm) in MCF-7/ADR cells treated as described in (i-j); Quantitative analysis of ADM intracellular concentration and TUNEL-positive cell ratio was shown in Fig.S4A and S4B, respectively

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