Fig. 6
US exposure reversed MDR in vivo. a ADM accumulation in MCF-7/ADR xenograft nude mice after US+ADM treatment with different acoustic intensities; N = 4; data are represented as mean ± s.d; *P < 0.05 vs. tumor tissue of 0 W/cm2 group; #P < 0.05 vs. peritumor muscle tissue of 0 W/cm2 group; (b-c) TUNEL staining in MCF-7/ADR xenograft after treatment with different US acoustic intensities; N = 4; data are represented as mean ± s.d; *P < 0.05 vs. tumor tissue of 0 W/cm2 group; #P < 0.05 vs. peritumor muscle tissue of 0 W/cm2 group; (d-e) Representative picture of MCF-7/ADR xenograft nude mice (d) and isolated tumors (e) after US+ADM treatment or ADM treatment on day 24; (f) Tumors volumes changes during the treatment; N = 6, data are represented as mean ± s.d; *P < 0.05; (g) Quantitative analysis of tumors weights after tumors were isolated; N = 6, *P < 0.05; (h) Tumor uptake of ADM after US+ADM treatment or ADM treatment on day 24; N = 6; data are represented as mean ± s.d; *P < 0.05; (i) TUNEL staining on respective tumor tissue to detect apoptotic cells; N = 6; data are represented as mean ± s.d; *P < 0.05; (j) Fluorescence signal of Ki-67 staining on respective tumor sections; scale bar = 50 μm; N = 6; data are represented as mean ± s.d; *P < 0.05