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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo

Fig. 7

The effect of US exposure on ROS-ZEB1-miR200c/34a-P-gp pathway in vivo. a ROS staining (green) of tumor tissue in MCF-7/ADR xenograft nude mice treated with ADM alone or US+ADM; scale bar = 50 μm; N = 6; data are represented as mean ± s.d; *P < 0.05; (b) MiR-200c/34a expression levels were quantified by qRT-PCR in MCF-7/ADR xenograft after US+ADM treatment or ADM treatment on day 24; N = 6; data are represented as mean ± s.d; *P < 0.05; (c-d) Detecting ZEB1 expression in respective tumor tissue by western blot (c) and immunochemistry (d, scale bar = 10 μm); N = 6; data are represented as mean ± s.d; *P < 0.05; (e-g) Detecting P-gp expression in respective tumor tissue by western blot (e), immunochemistry (f, scale bar = 10 μm), and immunofluorescence (g, scale bar = 100 μm); N = 6; data are represented as mean ± s.d; *P < 0.05; (h) Illustration of reversal of MDR mediated by US exposure in MDR cancer cells; Ultrasound exposure increases miR-200c/34a expression by promoting ROS generation. MiR-200c/34a overexpression directly or indirectly inhibits ZEB1 and P-gp expression. Down-regulation of ZEB1 in turn decreases its transcriptional repression on miR200c/34a. P-gp inhibition sensitizes MDR cells to MDR-associated drugs and increases the cytotoxicity of these chemotherapeutics

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