Fig. 4

THC is a main ISL metabolite and a Src inhibitor. a. Mass spectrum identifies THC and naringenin chalcone as main metabolites of ISL in H1299. b Cells were treated with vehicle, THC or PP1 for 24 h and then lysed for western blotting to detect Tyr416-phosphorylated Src, Src and β-actin with the respective antibodies. c Cells were treated with vehicle or THC for 24 h and then lysed for western blotting to detect Tyr421-phosphorylated cortactin, Tyr861-, Tyr925-phosphorylated FAK, cortactin, FAK and β-actin with the respective antibodies. d Cell-free system to determine the effect of THC and PP1 on Src kinase activity. Data are means ± SD. n = 3