Fig. 2

LMO3 knockdown suppresses the invasion and anoikis inhibition of HCC cells in vitro. a Expression of LMO3 in HCC cell lines, including HCCLM3, HepG2, Huh-7, MHCC-97H, MHCC-97 L, SK-Hep1, SMMC-7721, SNU-423 and SNU-449 cells. b and c The protein expression level of LMO3 in MHCC-97H (b) and SMMC-7721 (c) cells, which were infected with siRNA of LMO3 or NC. d Representative photos of invaded MHCC-97H cells infected with siRNA of LMO3 or NC. Statistical analysis of invaded MHCC-97H cells in the three groups is shown below. Scale bars: 10 μm. e Representative photos of invaded SMMC-7721 cells infected with siRNA of LMO3 or NC. Statistical analysis of invaded SMMC-7721 cells in the three groups is shown below. Scale bars: 10 μm. f Flow cytometry analysis of anoikis of MHCC-97H cells infected with siRNA of LMO3 or NC. Flow cytometry statistical analysis of apoptotic MHCC-97H cells in the two groups is shown below. g Flow cytometry analysis of anoikis of SMMC-7721 cells infected with siRNA of LMO3 or NC. Flow cytometry statistical analysis of apoptotic SMMC-7721 cells in the two groups is shown below. **P < 0.01, NS, no significant