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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: LMO3 promotes hepatocellular carcinoma invasion, metastasis and anoikis inhibition by directly interacting with LATS1 and suppressing Hippo signaling

Fig. 4

Knockdown of LMO3 increases the phosphorylation of YAP/LATS1 and decreases Rho GTPases activities. a and b Western blotting analysis of phospho-YAP, YAP, phospho-LATS1 and LATS1 in LMO3 knockdown and control MHCC-97H (a) or SMMC-7721 cells (b). Statistical analyses of phospho-YAP/YAP and phospho-LATS1/LATS1 densitometry are shown below. c and d MHCC-97H (c) or SMMC-7721 (d) cells were serum starved for 24Ā h and the activities of RhoA, Cdc42 and Rac1 were measured by pull-down assays in LMO3 knockdown and control cells. Statistical analyses of active-RhoA/total-RhoA and active-Cdc42/total-Cdc42 densitometry are shown below. e The mRNA levels of CTGF, ANKRD1 and CYR61 in LMO3 knockdown and control MHCC-97H cells. **Pā€‰<ā€‰0.01. f Western blotting analysis of CTGF, ANKRD1 and CYR61 in LMO3 knockdown and control MHCC-97H cells

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