Fig. 5

LMO3 directly interacts with LATS1, and recombinant LMO3 protein administration suppresses the phosphorylation of YAP/LATS1 and increases Rho GTPases activities. a Co-immunoprecipitation of LMO3 with LATS1 or YAP. Huh-7 cell lysates transfected with HA-tagged LMO3 or vector control were subjected to immunoprecipitation with anti-HA monoclonal antibody or control IgG, followed by immunoblotting with anti-LATS1 or YAP antibodies. The input control on the right panel shows the levels of transfected HA-LMO3 and LATS1 or YAP in HA-tagged LMO3 or vector control transfected cells. b and c Western blotting analysis of phospho-YAP, YAP, phospho-LATS1 and LATS1 in recombinant LMO3 (rLMO3) protein treated and control Huh-7 cells (b). Statistical analyses of phospho-YAP/YAP and phospho-LATS1/LATS1 densitometry are shown right (c). d and e Huh-7 cells were serum starved for 24 h and the activities of RhoA and Cdc42 were measured by pull-down assays in rLMO3 protein treated and control cells (d). Statistical analyses of active-RhoA/total-RhoA and active-Cdc42/total-Cdc42 densitometry are shown right (e). f The mRNA levels of CTGF, ANKRD1 and CYR61 in rLMO3 protein treated and control Huh-7 cells. *P < 0.05, **P < 0.01. g Western blotting analysis of CTGF, ANKRD1 and CYR61 in rLMO3 protein treated and control Huh-7 cells