Fig. 3
Apoptosis in AsPC-1, Capan-2 and BxPC-3 treated with erlotinib, nelfinavir or nitroxoline. Dot plots show representative experiments after 72 h of treatment with erlotinib, nelfinavir or nitroxoline in AsPC-1, Capan-2 and BxPC-3 cell lines (a, c and e, left). Values represented in the histograms are the means ± SD of at least two independent flow cytometry experiments (a, c and e, right). The expression of PARP and cleaved PARP in AsPC-1 (b), Capan-2 (d) and BxPC-3 (f) treated with the three drugs at the indicated concentrations for 48 h was analyzed by western blot. Ratios of cleaved:uncleaved PARP are indicated. *Statistically significant differences as compared to the vehicle (*p < 0.05; **p < 0.01; ***p < 0.001)