Fig. 6
Combinations of nelfinavir and nitroxoline, with or without erlotinib, induce apoptosis in AsPC-1, Capan-2 and BxPC-3. Dot plots show representative experiments analyzing apoptosis after 72 h of treatment with combinations of nitroxoline and nelfinavir, with or without erlotinib, in PC cell lines (a, c and e, left). Values represented in the histograms are the means ± SD of at least two independent flow cytometry experiments (a, c and e, right). The expression of PARP and cleaved PARP was analyzed by western blot in AsPC-1 (b), Capan-2 (d) and BxPC-3 (f) treated for 48 h with combination of nitroxoline and nelfinavir, with or without erlotinib. Ratios of cleaved:uncleaved PARP are indicated. *Statistically significant differences as compared to the corresponding value of vehicle (*p < 0.05; **p < 0.01)