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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: The LAT1 inhibitor JPH203 reduces growth of thyroid carcinoma in a fully immunocompetent mouse model

Fig. 2

JPH203 reduces proliferation of the ATC cell lines 8505c and SW1736 when EAA concentrations in medium were lowered to plasma level. Proliferation assays of two ATC cell lines (a) 8505c and (b) SW1736, the PTC cell line (c) K1 and (d) LNCaP cells as negative control were performed at different EAA concentrations (1X - 0.125X, see Table S1 for detailed composition). Cells were plated in sixplicate using normal growth medium, after overnight incubation cells were washed with PBS and medium containing specific EAA concentration was added. Cells were treated (DMSO/10 μM JPH203) and fixed after (a-c) 48 h or (d) 96 h (two population doubling times). Each condition was normalized to the proliferation of DMSO-treated 1X EAA. These results represent the average of three independent experiments. Statistical analysis was performed by using multiple t-tests, one per row, and Holm-Sidak correction for multiple comparisons. e Dose-response curves for JPH203 were conducted with three PTC cell lines (K1, KTC1, TPC-1) and three ATC cell lines (8505c, SW1736, Hth104) and LNCaP cells as a negative control using medium containing 0.125X EAA (see Table S1 for detailed composition). Cells were plated in triplicate using normal growth medium, after overnight incubation cells were washed with PBS and 0.125X EAA was added. Cells were treated with different concentrations of JPH203 between 2.5 nM and 50 μM or DMSO as control. 8505c, SW1736, Hth104, KTC1, TPC-1 and K1 cells were incubated for 48 h; LNCaP cells for 96 h (two population doubling times) and growth was normalized to DMSO. These results represent the average of three independent experiments.

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