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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways

Fig. 4

COMP facilitates EMT and MMP-2/9 expression in HCC cells. a The expression of EMT markers and transcription factors were determined via Western blot after treatment with various concentrations of rCOMP (as indicated) for 12 and 24 h. β-actin was used as a loading control. b The expression of E-cadherin (green) and vimentin (green) after treatment with rCOMP (2 μg /ml) were shown by immunofluorescence staining in both Hep-3B and SMMC-7721 cells. Representative images at × 400 magnification are shown. c The levels of MMP-2/9 in HCC cells after treatment with various concentrations of rCOMP (as indicated) for 12 and 24 h as detected by Western blot analysis. β-actin was used as a loading control. Western blot and IF analysis were independently repeated for three times with similar results

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