Fig. 4

Candidate miRNAs scanning, verification and its correlation with MLK7-AS1. (a,b, c) si-NC, si-MLK7-AS1–1, or − 2 was transfected into SKOV3, OVCAR3, and PEO1 cells, and then the expression levels of 5 candidate miRNAs were monitored using qRT-PCR. (d) MLK7-AS1 interacted with miR-375 by directly targeting. (e) miR-375 expression was significantly upregulated after MLK7-AS1 knockdown. (f) MLK7-AS1 mRNA expression was significantly downregulated in response to miR-375 overexpression while upregulated in response to miR-375 inhibition. (g, h, i) si-MLK7-AS1 or/and miR-375-inhibitor was transfected into SKOV3, OVCAR3 and PEO1 cells, and the transfection efficiency was verified using qRT-PCR. (j, k, l) MTT results showed that MLK7-AS1 were significantly downregulated in si-MLK7-AS1–1 or − 2 group compared to the si-NC group, while the effects were reversed in miR-375 inhibition cotransfected groups. The data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01