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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis

Fig. 6

MLK7-AS1 interacted with miR-375 and promoted progression of ovarian cancer cell through targeting YAP1. (a) YAP1 was predicted to be a target gene of miR-375 by TargetScan. (b) miR-375 regulated YAP1 expression by direct targeting using luciferase assay. (c) miR-375 levels were significantly decreased in human ovarian cancer tissues compared with adjacent non-tumor tissues. (d, e, f) The qRT-PCR assay showed that mRNA expressions of YAP1 were downregulated by miR-375 overexpression while upregulated by miR-375 inhibition in SKOV3, OVCAR3, and PEO1 cell lines. (g, h, i) Western blot assay showed that protein expressions of YAP1 were downregulated by miR-375 overexpression while upregulated by miR-375 inhibition in SKOV3, OVCAR3, and PEO1 cell lines. 1, mimics-NC; 2, miR-375-mimics; 3, inhibitor-NC; 4, miR-375-inhibitor. (j, k, l) The inhibitory function of MLK7-AS1 knockdown on YAP1 protein level was partially restored by the miR-375 inhibition. The data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01

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