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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis

Fig. 7

YAP1 promoted the EMT process by inducing Slug transcription. (a, b) E-cadherin and vimentin expression levels were determined when MLK7-AS1 knockdown or/and miR-375 inhibition in SKOV3, OVCAR3, and PEO1 cell lines using immunofluorescence. (c) By using gene sequence analysis, a putative binding site of the YAP1 in the promoter of Slug was found. (d, e) Slug protein and mRNA levels were increased in SKOV3, OVCAR3, and PEO1 cells when YAP1 was overexpressed. (f, g) Slug protein and mRNA levels were decreased in SKOV3, OVCAR3, and PEO1 cells when YAP1 was knockdown. (h, i, j) Slug expression levels were distinctly higher in tumor tissues than in adjacent non-tumor tissues using H&E, immunofluorescence, and qRT-PCR, respectively. (k) Slug expressions were determined when YAP1 was overexpressed or knockdown using immunofluorescence. (l, m, n) A RIP assay was performed to examine whether YAP1 and Slug were in the same RNA-induced silencing complex. (o) YAP1 regulated Slug expression by direct targeting using a luciferase assay. The data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01

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