Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Melanoma cell-secreted exosomal miR-155-5p induce proangiogenic switch of cancer-associated fibroblasts via SOCS1/JAK2/STAT3 signaling pathway

Fig. 4

Delivery of melanoma cell-secreted exosomal miR-155 into fibroblasts. a MiRNAs that might target SOCS1 were predicted with TargetScan, miRanda, and PicTar. b The predicted candidate miRNAs in B16- and B16F10-secreted exosomes were investigated by qRT-PCR. ** P < 0.01 vs. B16-secreted exosomes. Student’s t-tests. Independent experiments performed in triplicate. Values are expressed as means ± SEM. c Comparison of the miR-155 levels in NIH/3T3, B16, and B16F10 cells. d The levels of miR-155 in recipient NIH/3T3 cells after treatment with B16- and B16F10-secreted exosomes. * P < 0.05, ** P < 0.01, *** P < 0.001. Independent experiments performed in triplicate. Values are expressed as means ± SEM. One-way ANOVA and student’s t-tests. e Exosomes from B16F10 cells that were transfected with 40 nM FITC-tagged miR-155 were labeled with fluorescent dye PKH26 (left panel) and then applied to treat NIH/3T3 cells for 4 h, following by imaging under a fluorescence microscope. As negative controls, NIH/3T3 cells were incubated with non-labeled exosomes (exosomes were treated with PBS instead of PKH26, middle panel) or 40 nM naked FITC-tagged miR-155 (FITC-tagged miR-155 was added directly to the NIH/3T3 cells, right panel) for 4 h. FITC: FITC-tagged miR-155 (green), PKH26: PKH26-labeled exosomes (red), DAPI: cell nuclei (blue). Scale bar, 50 μm. DAPI: 4′6-diamidino-2-phenylindole. Exo: exosomes. FITC: fluorescein isothiocyanate. MiR-155: mmu-miR-155-5p. MiRNAs: microRNAs. SEM: standard error of the mean. SOCS1: suppressor of cytokine signaling 1

Back to article page