Fig. 1

Effect of pH treatments on MNI and EP morphology and C₁₆ intracellular distribution. MNI and EP cells were cultured with standard (ctr), pH 6.7 or pH 6.0 culture media for 24 h. Analysis of cell elongation (a) and perimeter (b) was performed by the determination of Feret’s diameter and cell perimeter of each cell displayed in Additional file 4. c MNI and EP cells were labeled with C₁₆ for 5 h and incubated at the indicated pHs for 24 h. Cells were afterwards fixed and analyzed by fluorescence under confocal microscopy. The nucleus was visualized with DAPI staining (blue). Scale bars, 10 μm. ***: p < 0.0001