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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules

Fig. 3

Analysis of C16-exo secreted in control or acidic conditions. a and b EP, MNI, PI and MI cells were treated with C16 (7 μm) for 5 h and then cultured in control or acid (pH 6.0 or pH 6.7) medium for 24 h. Hereafter conditioned medium was subjected to ultracentrifugation for exosome isolation. Fluorescent vesicles were then counted by FACS. The graph shows the number of secreted C16-exo per cell. c and d Western blot analysis of exosome for the presence of principal exosome markers (Alix, Tsg101 and CD81) and the absence of Calnexin, a marker of ER used as a negative control. Data shown are representative of three independent experiments. * p < 0.05

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