Fig. 2

Cotreatment with panobinostat and JQ1 markedly inhibits cell proliferation and induces apoptosis in GBM cells. a Cell proliferation analyses of GBM cells treated with DMSO, panobinostat, JQ1 or panobinostat/JQ1 for 24 h using an EdU incorporation FACS assay. Percentages of EdU+ cells are presented on the bar chart on the right. b Apoptosis analyses of GBM cells treated with DMSO, panobinostat, JQ1 or panobinostat/JQ1 for 48 h by an Annexin-V staining FACS assay. Percentages of Annexin-V+ cells are presented on the bar chart on the right. c Caspase 3/7 activity in GBM cells treated with DMSO, panobinostat, JQ1 or panobinostat/JQ1 at the indicated concentrations for 48 h using a Caspase-Glo assay. d Cytotoxicity of GBM cells treated with DMSO, panobinostat, JQ1 or panobinostat/JQ1 at the indicated concentrations for 48 h using a Cytotox-Glo assay. Blue or red asterisks indicate the P-values of panobinostat or JQ1 treatment alone compared with the combined drug treatment respectively. *p < 0.05, ** p < 0.01, *** p < 0.001, Students’ two-tailed t-test