Fig. 3

ARHGEF16 promotes migration and proliferation of glioma cells. a Detection of endogenous ARHGEF16 protein level in H4, U87, and U118 cells by western blotting. b Validation of glioma cell lines stably overexpressing ARHGEF16. ARHGEF16 protein level was compared between Flag-ARHGEF16 U87 or Flag-ARHGEF16 U118 cells and corresponding control cells. c, d Comparison of the migratory potential between ARHGEF16 U87 or ARHGEF16 U118 cells and their corresponding control cells with the transwell migration assay. n = 3, **, P < 0.01. e-h Comparison of the proliferative capacity of ARHGEF16 U87 or ARHGEF16 U118 cells and their corresponding control cells with EdU assay (e, f) and soft agar colony formation assay (g, h). n = 3, **, P < 0.01