Fig. 7

CKAP5 interacts with ARHGEF16 to regulate glioma cell migration and proliferation. a, b Examination of the interaction between ARHGEF16 and CKAP5 by immunoprecipitation (a) and GST pull-down (b). c Detection of ARHGEF16 and CKAP5 expression levels in Control+sh-Control, AREHGEF16 + sh-Control, and ARHGEF16 + sh-CKAP5 U87 cells by western blotting. d Migratory capacity of Control+sh-Control, AREHGEF16 + sh-Control, and ARHGEF16 + sh-CKAP5 U87 cells was determined with the transwell migration. n = 3, **, P < 0.01. e, f Soft agar colony formation assay (e) and EdU assay (f) to compare the proliferative capacity of the indicated U87 cells and their corresponding control cells. n = 3, **, P < 0.01. g The working model of this study. GLI2 directly activates ARHGEF16 transcription, which acts in conjunction with CKAP5 to promote glioma cell migration and proliferation induced by GLI2