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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway

Fig. 1

Overexpression of AFAP1-AS1 stimulates NPC cells migration and invasion of in vitro. a. AFAP1-AS1 levels were detected by qRT-PCR in 5-8F and HNE2 cells after transfection with the pcDNA3.1-AFAP1-AS1 (AFAP1-AS1) vector and the empty pcDNA3.1 vector (NC). b. Wound healing assays using 5-8F and HNE2 cells transfected with the pcDNA3.1-AFAP1-AS1 (AFAP1-AS1) or the pcDNA3.1 (NC) vectors. The relative ratio of wound closure per field is shown on the right. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001. c. Transwell analysis of 5-8F and HNE2 cells transfected with the pcDNA3.1-AFAP1-AS1 (AFAP1-AS1) or the pcDNA3.1 vectors (NC). The relative ratio of invasive cells per field is shown on the right. For all quantitative results, data are presented as the means and standard errors of the mean (± SEM) from three independent experiments. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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