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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway

Fig. 2

miR-423-5p could bind with the AFAP1-AS1 sequence. a. The expression level of AFAP1-AS1 was reduced by miR-423-5p mimics compared with negative controls (NC) in NPC cells transfected with miR-423-5p mimics or negative controls using the qRT-PCR method. b. The expression level of AFAP1-AS1 was induced by miR-423-5p inhibitors (423-5p In) in 5-8F and HNE2 cells transfected with miR-423-5p mimics or negative controls. c. Sequence alignment of human miR-423-5p within the AFAP1-AS1 sequence. The seed sequence of miR-423-5p matches the AFAP1-AS1 sequence. Mutations within the AFAP1-AS1 sequence. AFAP1-AS1 in the mutant luciferase reporter constructs are as shown. d. The pMIR-reporter-AFAP1-AS1 vector was used for the luciferase assays. 5-8F and HNE2 cells were transfected with miR-423-5p mimics or control and luciferase reporters containing the wide type or mutant AFAP1-AS1 sequence, as indicated. Firefly luciferase activity was normalized with Renilla luciferase activity. Relative luciferase activities are presented. Data represent three independent experiments in triplicate. e. Luciferase activity was measured in 5-8F and HNE2 cells cotransfected with miR-423-5p inhibitors, negative control, and the luciferase reporter constructs containing the wide type or mutant AFAP1-AS1 sequence, as indicated. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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