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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway

Fig. 3

The expression of miR-423-5p was negatively correlated with AFAP1-AS1 in NPC biopsies and inhibited NPC cell migration and invasion in vitro. a. miR-423-5p was lowly expressed in 32 NPC tissues compared with 13 nontumor nasopharyngeal epithelial (NPE) tissues and correlated with the TNM stages of NPC patients as indicated. b. AFAP1-AS1 was highly expressed in the same 32 NPC tissues compared with 13 nontumor tissues and tightly correlated with the TNM stages, lymphoma metastasis, and the T stage of NPC patients, as indicated. c. The expression of miR-423-5p was tightly negatively correlated with that of AFAP1-AS1 in the same 32 NPC tissues and 13 nontumor tissues as indicated. d. The invasion ability was measured in 5-8F and HNE2 cells after treatment with miR-423-5p mimics or negative control using wound healing assays. The relative ratio of wound closure per field is shown at the bottom. Scale bars, 100 μm. e. Transwell assays were performed to measure the migration ability of miR-423-5p mimics in 5-8F and HNE2 cells after treatment with miR-423-5p mimics or negative control. The relative ratio of invasive cells per field is shown at the bottom. Scale bars, 100 μm. f. Wound healing assays of 5-8F and HNE2 cells after treatment with miR-423-5p inhibitors or negative control. The relative ratio of wound closure per field is shown to the bottom. Scale bars, 100 μm. g. Transwell assays were performed in 5-8F and HNE2 cells after treatment with miR-423-5p inhibitors or negative control. The relative ratio of invasive cells per field is shown on the bottom. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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