Fig. 5

miR-423-5p directly targets the 3′-UTRs of RAB11B and LASP1. a and c. Schematic diagrams of miR-423-5p binding sites in the 3′-UTRs of the RAB11B (a) and LASP1 (c) mRNAs predicted using RNAhybrid software. b and d. Luciferase activity was measured in 5-8F and HNE2 cells after cotransfection with miR-423-5p mimics or inhibitors and luciferase reporter constructs containing either wild-type or mutated miR-423-5p binding sites from the RAB11B (b) or LASP1 (d) 3′-UTRs. miR-423-5p mimics attenuated luciferase activity from RAB11B-WT and LASP1-WT constructs, but not from RAB11B-mutant and LASP1-mutant constructs. *, p < 0.05; **, p < 0.01; ***, p < 0.001