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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway

Fig. 6

AFAP1-AS1 acts as a ceRNA to regulate FOSL2 gene expression through miR-423-5p. a. The expression of FOSL2 and its correlation with the TNM stages, lymphoma metastasis, and T stages in the same 32 NPC tissues and 13 nontumor tissues as indicated. b. The expression of FOSL2 was tightly positively correlated with that of AFAP1-AS1 in 32 NPC tissues and 13 nontumor tissues as indicated. c. The expression of FOSL2 was tightly negatively correlated with that of miR-423-5p in 32 NPC tissues and 13 nontumor tissues as indicated. d. Prediction of miR-423-5p binding sites in the FOSL2 transcript. The red nucleotides are complementary to the miR-423-5p seed sequences. e. The expression of FOSL2 was regulated by miR-423-5p at the mRNA and protein levels after transfection of miR-423-5p mimics, miR-423-5p inhibitors, or negative control in 5-8F and HNE2 cells. f. Luciferase activity was measured in 5-8F and HNE2 cells cotransfected with miR-423-5p inhibitors, miR-423-5p mimics, or negative control, and luciferase reporters containing WT or MT FOSL2 sequences, as indicated. g. The expression of FOSL2 was examined at the mRNA and protein levels in 5-8F and HNE2 cells after transfection with AFAP1-AS1 overexpression vector, or siAFAP1-AS1

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