Fig. 7

Functional analysis of FOSL2 in NPC cells. a. FOSL2 mRNA and protein levels were detected in 5-8F and HNE2 cells after transfection with the FOSL2 overexpression vector (FOSL2) or the empty vector (NC) by qRT-PCR and western blotting. b. Wound healing and transwell assays of 5-8F and HNE2 cells were conducted after transfection with pENTR-FOSL2 or pENTR empty vector. The relative ratio of wound closure per field is shown on the right. Scale bars, 100 μm. c. The expression of FOSL2 was examined at the mRNA and protein levels by qRT-PCR and western blotting in 5-8F and HNE2 cells after transfection with siFOSL2 or scrambled negative control siRNA. d. Wound healing and transwell assays were performed in 5-8F and HNE2 cells after treatment with siFOSL2 or scrambled siRNA control. The relative ratio of invasive cells per field is shown on the right. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001