Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: ATF3 inhibits the tumorigenesis and progression of hepatocellular carcinoma cells via upregulation of CYR61 expression

Fig. 1

ATF3 inhibited HCC cell proliferation in vitro and tumorigenesis in vivo. a As a quality control, in vitro ATF3 mRNA and protein expression was detected by qRT-PCR and western blot, respectively, in SK-Hep1, Li-7, MHCC-LM3 and MHCC-97H cells with ectopic ATF3 expression via the lentiviral vector (pWPXL). b The cell proliferative ability of HCC cells with ATF3 overexpression was inhibited as indicated by the CCK-8 assay. c The colony forming ability of HCC cells with ATF3 overexpression was reduced. d The percent of cell apoptosis with ATF3 overexpression in SK-Hep1 and Li-7 was increased. e Images of liver tissues collected from nude mice with tumor xenografts derived from SK-Hep1 (left top panel) and Li-7 (left bottom panel) cells with stable overexpression of ATF3. The livers with xenografts were weighed and compared (middle top and bottom panel). ATF3 protein expression in tissue samples of the xenografts was detected by western blot (right top and bottom panel). β-Actin was used as a loading control. The bar graphs in (a), (b), (c) and (d) represent the quantitative data from three independent experiments. Unpaired Student’s t-test was used for statistical analysis, and the data are shown as the mean ± S.D. *P < 0.05 and **P < 0.01

Back to article page