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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: ATF3 inhibits the tumorigenesis and progression of hepatocellular carcinoma cells via upregulation of CYR61 expression

Fig. 5

CYR61 suppressed HCC cell proliferation in vitro. a As a quality control, in vitro CYR61 mRNA and protein expression was detected by qRT-PCR and western blot, respectively, in MHCC-LM3 and MHCC-97H cells with ectopic CYR61 expression via the lentiviral vector (pWPXL) and SK-Hep1, Li-7 cells with silencing of CYR61. The cell proliferative ability of HCC cells with CYR61 overexpression and knockdown were examined by the CCK-8 assay (b) and the colony formation assay (c). d The percent of cell apoptosis with CYR61 overexpression was increased. The bar graphs in (a), (b), (c) and (d) represent the quantitative data from three independent experiments. Unpaired Student’s t-test was used for statistical analysis, and the data are shown as the mean ± S.D. *P < 0.05 and **P < 0.01

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