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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: ATF3 inhibits the tumorigenesis and progression of hepatocellular carcinoma cells via upregulation of CYR61 expression

Fig. 6

Knockdown CYR61 in HCC cells overexpressing ATF3 rescued the suppressive effects of ATF3 in vitro. a Western blot of CYR61 protein expression in ATF3-overexpressing HCC cells with CYR61 knockdown (shCYR61–1, − 2). β-Actin was used as a loading control. The influence of CYR61 knockdown on the inhibitory effects of ATF3 on cell growth (b), colony formation (d), migration (d) and invasion (e) in vitro were detected by the CCK-8 assay, colony formation assay, wound healing assay (scale bar, 200 μm) and transwell invasion assay (scale bar, 100 μm), respectively. The bar graphs in (b), (c), (d) and (e) represent quantitative data from three replicates. Data are shown as the mean ± S.D. from experiments with three replicates. *P < 0.05 and **P < 0.01

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