Fig. 2

Induction of apoptosis by each treatment in OS cells. Cells were treated with CAP (100 μM) and DDP (16.7 μM) alone or in combination for 24 h. Apoptosis in MG63, 143B and HOS cells was determined by flow cytometry after staining with Annexin V-FITC/PI (a, b). The expression levels of cell apoptosis-related proteins were measured by Western blotting (c). Statistical analyses of cytochrome C (d), cleaved caspase-3 (e), Bcl-2 (f), Bax (g) and survivin (h) expression in the three OS cell lines in response to the different treatments. The effects of CAP and DDP on alterations of the mitochondrial membrane potential (Δψm) in HOS cells were determined by JC-1 staining via fluorescence microscopy (i). The Δψm (red/green) alteration in MG63, 143B and HOS cells was determined by flow cytometry after staining with JC-1 (j). The quantitative data are shown as the mean ± SD of 3 independent experiments; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the control (CAP -, DDP-)