Fig. 3

Effects of each treatment on the cell cycle distribution in OS cells. Three OS cell lines (MG63, 143B and HOS) were treated with CAP (100 μM) and DDP (16.7 μM) alone or in combination for 24 h. Then, the cells were stained with PI and analyzed by flow cytometry (a, b). The expression levels of cell cycle-related proteins were measured by Western blotting (c). Statistical analyses of cyclin D1 (d), cyclin D3 (e), CDK2 (f), CDK4 (g), CDK6 (h), p18 (i), p21 (j) and p27 (k) in the three OS cell lines. The quantitative data are shown as the mean ± SD of 3 independent experiments; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the control (CAP-, DDP-)