Fig. 7

Effects of each treatment on the growth of human OS xenograft tumors. Nude mice bearing 143 human OS xenograft tumors were treated with CAP (20 mg/kg) and DDP (4 mg/kg) alone or in combination. The volumes of the xenograft tumors were measured at the indicated time points (a). The weight of each nude mouse was measured at the indicated time points (b). After the last treatment, the mice were sacrificed, and representative images of the subcutaneous tumor xenografts in the nude mice and the morphology of the tumors are presented (c). Tumors were collected, and the tumor weights were measured and compared (d). Xenograft tumors were sectioned and stained with PCNA and Ki67 via IHC (e). Statistical analyses of the expression of PCNA and Ki67 in different groups (f). Tumors were sectioned and stained with H&E, and representative histopathological images are presented (g). Effects of the different treatments on renal histology. Representative histological profiles of kidneys after the different treatments were detected by H&E staining (h). Effects of the different treatments on BUN and creatinine levels in mice (i). The quantitative data are shown as the mean ± SD of 5 independent experiments; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the control (CAP-, DDP-)