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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: HIF-2α promotes conversion to a stem cell phenotype and induces chemoresistance in breast cancer cells by activating Wnt and Notch pathways

Fig. 4

Overexpression of HIF-2α induces stem phenotype conversion and drug resistance by activating the Notch pathway. a Expression of Notch pathway-related proteins was detected in HIF-2α overexpressing MCF7 and MDA-MB-231 cells by western blot. b Expression of HIF-2α and Notch pathway-related proteins was detected after HIF-2α–overexpressing MCF7 cells were treated with L685,458 (2 μM) for 48 h by western blot. c Top: Cell viability was determined in HIF-2α–overexpressing MCF7 cells incubated with PTX and L685,458 (2 μM) for 48 h using the MTT assay. Bottom: Comparison of IC50 values and resistance index. d Cell apoptosis was measured after HIF-2α overexpressing MCF7 cells were incubated with PTX (3 nM) and L685,458 (2 μM) for 48 h by flow cytometry. Quantification values are shown. e Self-renew ability was analyzed after stable HIF-2α–cDNA transfected MCF7 cells were incubated with PTX (3 nM) and L685,458 (2 μM) for 48 h. f Expression of c-Myc, OCT4 and Nanog proteins was detected after HIF-2α–overexpressing MCF7 cells were treated with L685,458 (2 μM) for 48 h. Data are the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.0001 compared with the NC-cDNA group. #P < 0.05, ###P < 0.0001 compared with the HIF-2α-cDNA group

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