Fig. 1
Effect of Andro on cell proliferation in human breast cancer cells. a Breast cancer cells (MDA-MB-231, MCF-7, T47D, MDA-MB-361, and BT549) were cultured with the indicated concentrations of Andro for 48 h, and then the cell viability was determined by a CCK-8 assay. b The IC50 values of Andro for cell viability inhibition in breast cancer cells were determined. c MDA-MB-231 and MCF-7 cells were stained with CFSE and cultured with Andro for 48 h. Cell proliferation was determined by a BD Accuri C6 Flow Cytometer. d MDA-MB-231 and MCF-7 cells were cultured with Andro. The induced colony formation was analyzed, and the colony formation numbers were calculated. The data are presented as the mean ± SD of at least three separate experiments. (*P < 0.05, **P < 0.01, Andro treatment vs vehicle control groups)