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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Andrographolide inhibits breast cancer through suppressing COX-2 expression and angiogenesis via inactivation of p300 signaling and VEGF pathway

Fig. 7

Effect of Andro on VEGF-induced endothelial cell migration and invasion. Endothelial cell migration and invasion were analyzed by a wound-healing assay (a) and Transwell assay (b). HUVECs were plated, scratched and then incubated with Andro for 24 h, along with or without 50 ng/ml VEGF. Cell migration was measured by manual counting. Original magnifcation, 100× (a). b HUVECs were plated in Transwell pre-coated with matrigel. Cell migrated to the bottom of the membrane were counted by using an inverted microscope. Original magnifcation, 40×. c HUVECs were exposed to Andro for 0.5 h, and then stimulated with or without VEGF for 15 min. F-actin of cells was visualized by dyLightTM 554 palloidin staining and imaged by Leica confocal microscopy. d Andro inhibited VEGF-induced coflin phosphorylation in HUVECs using immunofluorescences staining with specifc antibody for phosphorylated coflin. Three independent experiments were performed. (##p < 0.01, VEGF-treated group vs. Solvent; **p < 0.05, **p < 0.01, VEGF-treated group vs. VEGF and Andro-treated group)

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