Fig. 8

Effect of Andro on tumor growth and tumor angiogenesis in a breast cancer mouse model. An orthotopic mouse model of human breast cancer MDA-MB-231 cells was used to evaluate the anti-tumor effect of Andro. The tumor pictures (a), tumor volumes (b) and total weights (c) were measured. d The expressions of COX-2 and CD31 in tumor samples were analyzed by immunohistochemistry and cofocol immunofluorescence, respectively. e The quantitative analysis of relative COX-2 expression and microvessel number were also performed. f The expression of COX-2 and CD31 proteins in tumor tissues was analyzed by Western blot. Data were represented as the mean ± S.D. (*P < 0.05, **P < 0.01, Andro treatment vs vehicle control groups, N = 5 mice/group. Magnification, 200×)