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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: MicroRNA-302a/d inhibits the self-renewal capability and cell cycle entry of liver cancer stem cells by targeting the E2F7/AKT axis

Fig. 1

Expression of CSC markers and miRNA-302 family in LCSCs. qRT-PCR (a) and Western blot (b) to quantify CD133 and EpCAM level in adherent and tumor spheres of HepG2 and Huh7 cells after 3 days cultured in stem cell medium containing EGF and bFGF. GAPDH was used as loading control. c, MiRNAs microarray analysis to identify key miRNA molecules implicated in LCSCs maintenance. qRT-PCR to validate the miRNA-302a (d) and -302d (e) level in adherent and tumor spheres of HepG2 and Huh7 cells after 3 days cultured in stem cell medium containing EGF and bFGF. f, Western blot to quantify CD133 and EpCAM level in tumor spheres of HepG2 and Huh7 cells at Day 7 cultured in stem cell medium and differentiated liver cancer cells. qRT-PCR to quantify the miRNA-302a (g) and -302d (h) mRNA level in adherent and tumor spheres of HepG2 and Huh7 cells at Day 7 cultured in stem cell medium and differentiated liver cancer cells. Data shown are the means ± SD of three independent experiments. Statistical analyses were performed with one-way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001 vs. normal)

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