Fig. 2
Biological role of miRNA-302a/d in HCC in vitro. qRT-PCR measurement of the levels of miRNA-302a/d mRNA in HepG2 and Huh7 cells before and after knockdown (a) or overexpression (b) of miRNA-302a/d. Data shown are the means ± SD of three independent experiments. c, Tumor sphere assays of HepG2 and Huh7 cells before and after knock down of miRNA-302a/d. d, HepG2 and Huh7 cell counts in 96-well plate after transfection with negative control or miRNA-302a/d knockdown plasmids at the indicated day. e, HepG2 and Huh7 cell apoptosis was examined by flow cytometry analysis before and after knockdown of miRNA-302a/d. f, Tumor sphere assays of HepG2 and Huh7 cells before and after overexpression of miRNA-302a/d. g, HepG2 and Huh7 cell counts in 96-well plate after transfection with negative control or miRNA-302a/d overexpression plasmids at the indicated day. h, HepG2 and Huh7 cell apoptosis was examined by flow cytometry analysis before and after overexpression of miRNA-302a/d. Statistical analyses were performed with one-way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001 vs. normal)