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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MicroRNA-302a/d inhibits the self-renewal capability and cell cycle entry of liver cancer stem cells by targeting the E2F7/AKT axis

Fig. 5

The effect of miRNA-302a/d and/or E2F7 on AKT1-cyclin D1 signaling and downstream cell cycle. a, Cell cycle quantitative analysis of HepG2 cells transfected with the miRNA-302a/d knockdown or the control vector. b, Western blot to quantify the effect of miRNA-302a/d knockdown on AKT1-cyclin D1 signaling in HepG2 cells. c, Cell cycle quantitative analysis of HepG2 cells transfected with the miRNA-302a/d overexpression or the control vector. d, Western blot to quantify the effect of miRNA-302a/d overexpression on AKT1-cyclin D1 signaling in HepG2 cells. e, Western blot to quantify the effect of miRNA-302a/d and E2F7 overexpression on AKT1-cyclin D1 signaling in HepG2 cells. f, Cell cycle quantitative analysis of HepG2 cells transfected with the miRNA-302a/d and E2F7 overexpression or the control vector. g, Cell cycle quantitative analysis of CD133+EpCAM+ HepG2 cells transfected with the miRNA-302a/d and/or E2F7 overexpression or the control vector. h, Western blot to quantify the effect of miRNA-302a/d and/or E2F7 on protein levels of intranuclear β-catenin level in HepG2 cells. Western blot to quantify the effect of AKT1 (i) and Cyclin D1 (j) on E2F7 protein level in HepG2 and Huh7 cells. Western blot to quantify the effect of AKT1 (k) and Cyclin D1 (l) on intranuclear β-catenin level in HepG2 and Huh7 cells

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