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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Apigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects

Fig. 4

Apigenin and curcumin attenuate IFN-γ-induced STAT1 phosphorylation in human melanoma cells. a-c, A375, A2058, and RPMI-7951 cells were incubated with DMSO, curcumin (25 μM), or apigenin (30 μM) for 4 h and then treated with IFN-γ (10 ng/ml) for indicated times. The levels of STAT1 phosphorylation at Tyr701 and total STAT1 were detected by Western blot analysis. GAPDH was used as a loading control. Line charts on the right show the quantitation data of pSTAT1 by comparing to the levels with 1 min IFN-γ stimulation from the control group (100%) of each cell line. Data were presented as the mean ± S.D. from three independent experiments

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