Fig. 4

The expression of miR-31-5p correlates with the PARP1 when treated with Oxaliplatin-based chemotherapeutics and the intracellular Oxaliplatin content is increased with miR-31-5p reintroduction. a Expression levels of PARP1 were determined in miR-VC or miR-31-5p HCC with Oxaliplatin for 24 h, 48 h and 72 h. Across all time points, it is evident that levels of PARP1 decreased in miR-31-5p-positive cells. b Representative western blot for PARP1 with Oxaliplatin treatment. The confirmation of a reduction in PARP1 with miR-31-5p, with an increase in PARP1 evident in the miR-31-suppressed Huh7 cell line. c The effect of PARP1 level on cell resistance to OXA and OXA accumulation used with western blot. d Immunofluorescent indicated that the miR-31-5p may prevents the nuclear localization of PARP1. e ICP-MS analysis of Hep3B and Huh7-transfected cell lines treated with Oxaliplatin for 24 h. There is a significant increase in the levels of Oxaliplatin in Hep3B miR-31-5p cells compared to miR-VC equivalent, but there is no change in overall Oxaliplatin levels with miR-31-5p suppression. Data are presented as the mean ± SEM. *p < 0.05; **p < 0.01; ns, not significant