Fig. 5

miR-31-5p affects the Oxaliplatin content of specific organelles within the intracellular environment. a ICP-MS analysis indicated a significant difference in Oxaliplatin within the nuclear fraction of miR-31-5p-expressing cells following Oxaliplatin treatment for 24 h. A trend toward an decrease concentration within the Hep3B miR-31-5p. And there is not significant with the Huh7 Zip -miR-31-5p. b Isolation of the lysosomal fraction Oxaliplatin treatment for 24 h illustrated a trend toward increased Oxaliplatin concentration with miR-31-5p reintroduction; however, this is not significant (ns = not significant) .Data are presented as the mean ± SEM.*p < 0.05; **p < 0.01. cNuclear fraction suggests that miR-31-5p can promote PARP1 regaining to nuclear by western blot after OXA treatment. PCNA was used as a loading control