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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: RETRACTED ARTICLE: MicroRNA-31-5p regulates chemosensitivity by preventing the nuclear location of PARP1 in hepatocellular carcinoma

Fig. 7

Lysosomally bound ABCB9 is upregulated with miR-31-5p re-expression and PARP1 interacts ABCB9 inhibits its nuclear localization in HCC cells. a miR-31-5p prevents the nuclear migration of PARP-1. Hep3B and Huh7 cells were transfected with miR-31-5p. PARP-1 localization was detected by protein blotting. Immunoblotting was also performed using an anti-PCNA antibody as an internal control for nuclear loading. b Cellular localization of PARP-1. Hep3B miR-31-5p reintroduction illustrated PARP1 to cytoplasm. But, when treated with Oxaliplatin, PARP1 were regaining to nuclear. This were supported by immunofluorescence. Localization of eIF4E was also performed to show the specificity of miR-31-5p and Oxaliplatin towards PARP-1. c Expression levels of the drug influx transporter abcb9 were analyzed via qPCR. There is a significantly greater relative expression level of ABCB9 in miR-31-5p transfected cells compared to the miR-VC-transfected equivalent. RQ relates to relative fold change. d-f Representative western blot, qPCR and immunofluorescent illustrating an increase in ABCB9 expression level with miR-31-5p re-expression, with no apparent change in the lysosomal marker LAMP1. g PARP1 and ABCB9 form a complex in Hep3B cells which treatment with Oxaliplatin or not following transfected with miR-VC or miR-31-5p. Then separating and extracting their nuclear proteins for coimmunoprecipitation (IP) with respective antibodies

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