Fig. 6

Inhibition of differentiation by UCHL1 dysregulation is associated with AKT and ERK1/2 signaling. a shNC and shUCHL1 SH-SY5Y cells were treated with DMSO or RA (10 μM) for 3 and 5 days. b shNC and shUCHL1 SK-N-BE (2) cells were treated with DMSO or RA (10 μM) for 7 days. The protein expression of phospho-AKT (Ser473), AKT, phospho-ERK1/2 (Thr202/Tyr204), ERK1/2 and UCHL1 were measured by immunoblotting analysis in the indicated time. The densitometry of the bands was quantified using ImageJ software, and AKT and ERK1/2 were used as controls, respectively