Fig. 8
From: Lentinan inhibits tumor angiogenesis via interferon γ and in a T cell independent manner
Anti-IFN-γ antibody treatments reverse the effects of LNT treatments on tumor vessels in LAP0297 lung cancer. LAP0297 tumor bearing mice were prepared as described in Fig. 1. When tumors reached 4 × 4 mm in diameter, mice were randomly divided into 4 groups and received i.p. injection of HRPN, LNT (1.0 mg/kg), anti-IFN-γ antibody (250 μg/mouse) or LNT plus anti-IFN-γ antibody for 10 days. Tumor vascular parameters were determined as described in Fig. 2. a and b The growth curves and weight of LAP0297 tumors treated with HRPN, LNT, anti-IFN-γ antibody, or the combination of LNT and anti-IFN-γ antibody. c Representative figures showing tumor vascular function and vessel density. d The quantifications of Hoechst33342 perfused tumor areas and tumor vessel density. ns: no significantly different, * p < 0.05, ** p < 0.01, *** p < 0.001