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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Abnormally elevated USP37 expression in breast cancer stem cells regulates stemness, epithelial-mesenchymal transition and cisplatin sensitivity

Fig. 3

Effect of USP37 expression on breast cancer cell EMT, migration and invasion. a MCF-7 and MDA-MB-231 cells were transfected with USP37 siRNAs or NC siRNA for 48 h. Western blotting analysis of USP37 knockdown efficiency in MCF-7 and MDA-MB-231 cells. Relative expression levels were analyzed by Image-Pro Plus 6.0 software. b Real-time quantitative PCR analysis of USP37 knockdown efficiency in MCF-7 and MDA-MB-231 cells. c, d USP37 knockdown significantly decreased N-cadherin, Snail1 and Vimentin as well as increased E-cadherin expression in MCF-7 and MDA-MB-231 cells. e Immunofluorescence staining of E-cadherin and N-cadherin after transfection with NC siRNA or USP37 siRNA#2 (Scale bar: 50 μm). f, g Representative images of migration assays for MCF-7 and MDA-MB-231 cells after downregulation of USP37 are shown at 12, 24 and 48 h (Scale bar: 200 μm). **P < 0.01, ***P < 0.001. h Representative images of Matrigel-based Transwell assay for MCF-7 and MDA-MB-231 cells after USP37 knockdown are shown at the 48 h time point (Scale bar: 200 μm).*P < 0.05. i MCF-7 cells were transfected with control plasmid (CTL) and a USP37 overexpression plasmid (USP37) for 48 h. USP37 upregulation significantly decreased E-cadherin and increased N-cadherin, Snail1 and Vimentin expressions in both MCF-7 and MDA-MB-231 cells. j Immunofluorescence staining of E-cadherin and N-cadherin after USP37 upregulation (Scale bar: 50 μm). k, l Representative images of migration and Matrigel-based Transwell assay for MCF-7 and MDA-MB-231 cells after USP37 upregulation (Scale bar: 200 μm).*P < 0.05, **P < 0.01, ***P < 0.001

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