Fig. 2

Tunicamycin preferentially leads MDR GC cells to death in a time/dose-dependent manner via inducing ER stress. a Survival of GC cells after Tu treatment for 24/48/72 h assayed by CCK-8. ns, non-significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (green, VCR versus 7901; red, ADR versus 7901). b Expressions of ER stress-associated proteins in GC cells after Tu treatment for 48 h. All proteins were normalized to β-actin. c Expressions of Bip and CHOP in SGC7901 and SGC7901/ADR detected by immunofluorescence (IF) after Tu (0.8 μg/ml) treatment for 48 h. (400 ×; scale bar, 50 μm)