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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Tunicamycin specifically aggravates ER stress and overcomes chemoresistance in multidrug-resistant gastric cancer cells by inhibiting N-glycosylation

Fig. 5

Tu-induced autophagy antagonizes the chemosensitizing effects of Tu on MDR GC cells. a Expressions of LC3 II and P62 in GC cells after Tu treatment (0.8 μg/ml) for 48 h, as detected by WB. All proteins were normalized to β-actin. b Expression of LC3 II in GC cells after Tu treatment (0.8 μg/ml) for 48 h, as detected by IF. (400 ×; scale bar, 50 μm.) c The effects of autophagy blockade on the survival of GC cells assayed by CCK-8. HCQ, hydroxychloroquine; Tu, 0.8 μg/ml; HCQ, 25 μM; Adr, 0.25 μg/ml for 7901, 8 μg/ml for ADR. Cells were subjected to treatments for 48 h before the CCK-8 assay. ****P < 0.0001. d The effects of autophagy blockade on the apoptosis of GC cells, as determined by flow cytometry. The treatments were the same as those in 5c. ****P < 0.0001. e Expression levels of proteins involved in autophagy and apoptosis signaling in GC cells after treatments for 48 h. The treatments were the same as those in 5c. All proteins were normalized to β-actin

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