Fig. 4

CircRNA_102171 interacts with CTNNBIP1. a RNA-FISH showed that circRNA_102171 was located in the nucleus of PTC cells. b CTNNBIP1 was a potential interactive protein of circRNA_102171 according to RNA-pulldown followed by silver staining and mass spectrum analysis. CircRNA_102171 and anti-sense were labeled with biotin. c RNA-IP showed that anti-CTNNBIP1 precipitated circRNA_102171 in cell lysates. d RNA-pull down showed that biotin-labeled circRNA_102171 precipitated CTNNBIP1 in cell lysates. e RNA-EMSA indicated that circRNA_102171 directly interacted with CTNNBIP1. ***p < 0.001